WormBase Tree Display for Transgene: WBTransgene00008000
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| WBTransgene00008000 | Public_name | wgIs87 | |
|---|---|---|---|
| Summary | [ceh-6::TGF(9H1)::GFP::TY1::3xFLAG inserted into fosmid WRM0617dE06 as C-terminal protein fusion] | ||
| Synonym | [P000007_A02 protein::TY1-eGFP-3XFLAG] | ||
| [pes-1::TY1::EGFP::3xFLAG + unc-119(+)] | |||
| [pes-1::TY1::EGFP::FLAG; unc-119(+)] | |||
| Construction | Construct | WBCnstr00007749 | |
| Coinjection_other | unc-119(+) | ||
| Integration_method | Particle_bombardment | ||
| Construction_summary | This transgenic strain carries a 30-40 kb fosmid containing the entire transcription factor locus along with flanking regions. The TF has been tagged in frame with a dual GFP:3xFLAG tag at its C terminus (Sarov et al. 2010; Zhong et al. 2010). | ||
| A GFP-3XFLAG tag was introduced at the C-terminus of each transcription factor's coding region in a genomic fosmid clone by recombineering (Sarov et al. 2006). An unc-119 rescue marker was introduced by recombineering into the fosmid backbone and integrated transgenic strains were made by bombardment. Amplified from WRM0633dB02. | |||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr11553 | |
| Expr15599 | |||
| Expr1200174 | |||
| Associated_with | Strain | WBStrain00029996 | |
| WBStrain00033659 | |||
| Reference | WBPaper00037946 | ||
| WBPaper00040986 | |||
| WBPaper00046510 | |||
| WBPaper00047042 | |||
| WBPaper00060123 | |||
| WBPaper00060124 | |||
| Species | Caenorhabditis elegans |
