WormBase Tree Display for Transgene: WBTransgene00008382
expand all nodes | collapse all nodes | view schema
WBTransgene00008382 | Public_name | mcEx534 | |
---|---|---|---|
Summary | [Pgei-16A::GEI-16::GFP] | ||
Synonym | gei-16A | ||
Construction | Construct | WBCnstr00008091 | |
Coinjection | WBCnstr00004720 | ||
Construction_summary | This translational GFP reporter for isoform A was created by amplifying genomic DNA from the cosmid T17H7 with the following primers: construct A, 5'-accggtctgcaaacccttcctgtagc and 5'-accggtcgtcgaccaatatgttttttgagc. Promoters were directionally cloned into pPD95.75 with SphI and KpnI enzymes; cDNAs were placed between promoters and the GFP coding sequence by digestion with AgeI. and was inserted at the AgeI site of the vector pPD95.75 (Addgene). | ||
Genetic_information | Extrachromosomal | ||
Used_for | Expr_pattern | Expr11568 | |
Reference | WBPaper00037850 | ||
Species | Caenorhabditis elegans |