WormBase Tree Display for Transgene: WBTransgene00008384
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| WBTransgene00008384 | Public_name | mcEx539 | |
|---|---|---|---|
| Summary | [Pgei-16E::GEI-16::GFP] | ||
| Synonym | gei-16E | ||
| Construction | Construct | WBCnstr00008093 | |
| Coinjection | WBCnstr00004720 | ||
| Construction_summary | This translational GFP reporter for isoforms E/F/I was created by PCR fusion, using a promoter PCR product and a GFP PCR product using the primers 5'-tgcaatacgtccacctgaaa and 5'-gagtcgacctgcaggcatgcaagctacgacgacgtctatcatcattcag (promoter and coding sequence), 5'-agcttgcatgcctgcaggtcgact and 5'-tctgtgcggtatttcacacc (GFP coding sequence and unc-54 3'UTR from the plasmid pPD95.75). The final product was PCR products were inserted at the AgeI site of the vector pPD95.75 (Addgene). | ||
| Genetic_information | Extrachromosomal | ||
| Used_for | Expr_pattern | Expr11571 | |
| Reference | WBPaper00037850 | ||
| Species | Caenorhabditis elegans |
