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WormBase Tree Display for Transgene: WBTransgene00008688

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Name Class

WBTransgene00008688Public_nameoxEx593
Summary[Pmca-3::GFP]
ConstructionConstructWBCnstr00008391
Construction_summaryClone = pEB73. This construct consists of a 2.5-kb region upstream of mca-3 ATG, amplified from genomic wild-type DNA with oligos oEB232 [AATTCTGCAGCACAATGGCTACAGTAGCC] and oEB237 [TTCTACCGGTACCCGAAGCTCCTCCAGTGATG]. These primers append PstI and AgeI restriction sites, respectively. The promoter fragment was then inserted into a PstI and AgeI digested Fire lab vector pPD95.75 to produce a transcriptional GFP fusion.
Genetic_informationExtrachromosomal
Used_forExpr_patternExpr8320
Expr12128
ReferenceWBPaper00029172
SpeciesCaenorhabditis elegans