WormBase Tree Display for Transgene: WBTransgene00008983
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| WBTransgene00008983 | Public_name | cgIs55 |
|---|---|---|
| Summary | [Pric-19::PrP; Pric-19::GFP] | |
| Construction (2) | ||
| Construction_summary | The coding sequence for mouse PrP(1-254) carrying a human sequence-based 3F4 epitope was PCR amplified from the PrP1-254-mPrP1,28 using the following primers: 5'-GCG CGG CTA GCA TGG CGA ACC TTG GCT ACT GG-3' (forward), 5'-GCG CGG AGC TCT CAT CCC ACG ATC AGG AAG ATG A-3' (reverse). The resulting PCR products were digested with NheI/SacI and ligated to a plasmid that had been predigested with NheI/SacI: pRB490, containing the dopamine neuronal specific promoter Pdat-1,29 to create Pdat-1::PrP(1-254). | |
| Genetic_information (2) | ||
| Reference | WBPaper00037809 | |
| Species | Caenorhabditis elegans |
