WormBase Tree Display for Transgene: WBTransgene00009203
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WBTransgene00009203 | Public_name | rmIs227 | |||||
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Summary | [pF25B3.3::AT3v1-1q14::yfp] | ||||||
Synonym | AT3q14 | ||||||
Q14 | |||||||
Construction | Construct | WBCnstr00008890 | |||||
Integration_method | Gamma_irradiation | ||||||
Construction_summary | Pan-neuronal ATXN3 expression was achieved by cloning AT3var1-1 cDNA into the PF25B3.3Q0::YFP plasmid (32). Full-length ATXN3 cDNA with different polyQ lengths was generated by PCR using oligonucleotides containing restriction sites for BamH I and pBluescriptIISK(+)::MJD1-1q14 as templates. ATXN3 amplicons were then digested and ligated into the BamH I sites of PF25B3.3Q0::YFP, generating PF25B3.3AT3v1-1q(n)::YFP. | ||||||
Genetic_information | Integrated | ||||||
Phenotype_not_observed | WBPhenotype:0000039 | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Animals were similar to WT N2 animals. | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | ATXN3 with poly-Q(14) expansion | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0001206 | Paper_evidence | WBPaper00038396 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Animals were similar to WT N2 animals. | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | ATXN3 with poly-Q(14) expansion | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0001472 | Paper_evidence | WBPaper00038396 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Animals were similar to WT N2 animals. | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_other | ATXN3 with poly-Q(14) expansion | Paper_evidence | WBPaper00038396 | ||||
Curator_confirmed | WBPerson712 | ||||||
Reference | WBPaper00038396 | ||||||
Species | Caenorhabditis elegans |