WormBase Tree Display for Transgene: WBTransgene00009338
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WBTransgene00009338 | Public_name | WBPaper00039785Ex1 | ||||||
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Summary | [Ppcm-1::PCM-1] | |||||||
Synonym | PL51 | |||||||
WBPaper00039785_Ex1 | ||||||||
Construction | Construct | WBCnstr00009019 | ||||||
Construction_summary | The plasmids used for creating the transgenic strain (in a pcm-1 mutant background) also contained the C10F3.4/mcp-1 gene, which overlaps the pcm-1 gene in the C. elegans genome in antiparallel orientation. | |||||||
Genetic_information | Extrachromosomal | |||||||
Phenotype | WBPhenotype:0000039 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00003954 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00039785 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000125 | Paper_evidence | WBPaper00039785 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Three to seven-fold higher isoaspartyl methyltransferase activities were measured in transgenic PL51 [pcm-1p::PCM-1]; pcm-1(qa201) worm extracts compared to wildtype N2 extracts prepared from animals grown in liquid culture. | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00003954 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000135 | Paper_evidence | WBPaper00039785 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Relative transcript levels of DAF-16 regulated genes sod-3, hsp-12.6, and dod-3 were elevated compared to wild-type; levels of daf-16 itself was no different from wild-type. Transcript levels of DAF-16 down-regulated genes were not different from wild-type. | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00003954 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000295 | Paper_evidence | WBPaper00039785 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Day 1 adults displayed increased thermotolerance. | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00003954 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_not_observed | WBPhenotype:0001644 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | In PCM-1 overexpressing (PL51) extracts, there was no apparent decrease in isoaspartyl damage compared to wild-type levels. | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_gene | WBGene00003954 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Associated_with | Strain | WBStrain00044729 | ||||||
Reference | WBPaper00039785 | |||||||
Species | Caenorhabditis elegans |