Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Transgene: WBTransgene00009338

expand all nodes | collapse all nodes | view schema

Name Class

WBTransgene00009338Public_nameWBPaper00039785Ex1
Summary[Ppcm-1::PCM-1]
SynonymPL51
WBPaper00039785_Ex1
ConstructionConstructWBCnstr00009019
Construction_summaryThe plasmids used for creating the transgenic strain (in a pcm-1 mutant background) also contained the C10F3.4/mcp-1 gene, which overlaps the pcm-1 gene in the C. elegans genome in antiparallel orientation.
Genetic_informationExtrachromosomal
PhenotypeWBPhenotype:0000039Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Caused_by_geneWBGene00003954Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Phenotype_assayTemperature25Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
WBPhenotype:0000125Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
RemarkThree to seven-fold higher isoaspartyl methyltransferase activities were measured in transgenic PL51 [pcm-1p::PCM-1]; pcm-1(qa201) worm extracts compared to wildtype N2 extracts prepared from animals grown in liquid culture.Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Caused_by_geneWBGene00003954Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
WBPhenotype:0000135Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
RemarkRelative transcript levels of DAF-16 regulated genes sod-3, hsp-12.6, and dod-3 were elevated compared to wild-type; levels of daf-16 itself was no different from wild-type. Transcript levels of DAF-16 down-regulated genes were not different from wild-type.Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Caused_by_geneWBGene00003954Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
WBPhenotype:0000295Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
RemarkDay 1 adults displayed increased thermotolerance.Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Caused_by_geneWBGene00003954Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0001644Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
RemarkIn PCM-1 overexpressing (PL51) extracts, there was no apparent decrease in isoaspartyl damage compared to wild-type levels.Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Caused_by_geneWBGene00003954Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Associated_withStrainWBStrain00044729
ReferenceWBPaper00039785
SpeciesCaenorhabditis elegans