WormBase Tree Display for Transgene: WBTransgene00009339
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WBTransgene00009339 | Public_name | WBPaper00039785Ex2 | ||||||
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Summary | [Ppcm-1::PCM-1(G88V and G90V)] | |||||||
Synonym | PL54 | |||||||
WBPaper00039785_Ex2 | ||||||||
Construction | Construct | WBCnstr00009020 | ||||||
Construction_summary | The plasmids used for creating the transgenic strain (in a pcm-1 mutant background) also contained the C10F3.4/mcp-1 gene, which overlaps the pcm-1 gene in the C. elegans genome in antiparallel orientation. | |||||||
Genetic_information | Extrachromosomal | |||||||
Phenotype | WBPhenotype:0000124 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Extracts prepared from PL54 nematodes did not demonstrate any methyltransferase activity. | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | mutant PCM-1 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000135 | Paper_evidence | WBPaper00039785 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Relative transcript levels of DAF-16 regulated hsp-12.6 were elevated compared to wild-type; levels of daf-16 itself was no different from wild-type. Transcript levels of DAF-16 down-regulated genes dod-22 and dod-22 were not significantly elevated compared to wild-type. | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | mutant PCM-1 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_not_observed | WBPhenotype:0000039 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Worms overexpressing this mutated form of PCM-1 in a pcm-1(qa201) null background did not exhibit a life span different from N2. | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | mutant PCM-1 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Temperature | 25 | Paper_evidence | WBPaper00039785 | ||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000295 | Paper_evidence | WBPaper00039785 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | PL54 animals did not show significantly increased thermotolerance at any noted time point. | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | mutant PCM-1 | Paper_evidence | WBPaper00039785 | |||||
Curator_confirmed | WBPerson712 | |||||||
Associated_with | Strain | WBStrain00044730 | ||||||
Reference | WBPaper00039785 | |||||||
Species | Caenorhabditis elegans |