Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Transgene: WBTransgene00009339

expand all nodes | collapse all nodes | view schema

Name Class

WBTransgene00009339Public_nameWBPaper00039785Ex2
Summary[Ppcm-1::PCM-1(G88V and G90V)]
SynonymPL54
WBPaper00039785_Ex2
ConstructionConstructWBCnstr00009020
Construction_summaryThe plasmids used for creating the transgenic strain (in a pcm-1 mutant background) also contained the C10F3.4/mcp-1 gene, which overlaps the pcm-1 gene in the C. elegans genome in antiparallel orientation.
Genetic_informationExtrachromosomal
PhenotypeWBPhenotype:0000124Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
RemarkExtracts prepared from PL54 nematodes did not demonstrate any methyltransferase activity.Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Caused_by_othermutant PCM-1Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
WBPhenotype:0000135Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
RemarkRelative transcript levels of DAF-16 regulated hsp-12.6 were elevated compared to wild-type; levels of daf-16 itself was no different from wild-type. Transcript levels of DAF-16 down-regulated genes dod-22 and dod-22 were not significantly elevated compared to wild-type.Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Caused_by_othermutant PCM-1Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000039Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
RemarkWorms overexpressing this mutated form of PCM-1 in a pcm-1(qa201) null background did not exhibit a life span different from N2.Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Caused_by_othermutant PCM-1Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Phenotype_assayTemperature25Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
WBPhenotype:0000295Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
RemarkPL54 animals did not show significantly increased thermotolerance at any noted time point.Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Caused_by_othermutant PCM-1Paper_evidenceWBPaper00039785
Curator_confirmedWBPerson712
Associated_withStrainWBStrain00044730
ReferenceWBPaper00039785
SpeciesCaenorhabditis elegans