WormBase Tree Display for Transgene: WBTransgene00009391

WBTransgene00009391 Public_name: geIs3
  Summary: [sir-2.1(+) + rol-6(su1006)]
  Synonym: [Pint sir-2.1(+) rol-6(su1006)]
    [Psir-2.1(+), rol-6(su1006)]
  Construction: Construct: WBCnstr00009072
    Integration_method: Particle_bombardment
  Construction_summary: LG100 geIs3 (formerly known as geIn3), was out-crossed four times to wild-type N2 worms along with geIs101, an integrated rol-6(su1006) transgene control allele, creating LG269 and LG268, respectively.
    geIs3 contains only 500 bases of DNA upstream of the start of sir-2.1 and therefore lacks P0, an upstream operon promoter, instead, this construct only has the intergenic promoter, Pint.
    Pint is a cryptic intergenic promoter that drives sir-2.1 expression in neurons and the hypodermis.
  Genetic_information: Integrated
    Phenotype: WBPhenotype:0000061 Paper_evidence: WBPaper00040228
        Curator_confirmed: WBPerson712
        Remark: LG389 (geIs3 Dyf+), has a shorter mean lifespan relative to its Dyf- progenitor, LG269, but is still long-lived relative to the similarly outcrossed control strain LG390 geIs101. Further outcross of geIs3 resulted in lines (LG391-LG394) with an increase in lifespan compared to similarly outcrossed control lines. Paper_evidence: WBPaper00040228
            Curator_confirmed: WBPerson712
        Caused_by_gene: WBGene00004800 Paper_evidence: WBPaper00040228
            Curator_confirmed: WBPerson712
      WBPhenotype:0000136 Paper_evidence: WBPaper00040228
        Curator_confirmed: WBPerson712
        Remark: All strains harbouring geIs3 show increases in sir-2.1 mRNA of 10-30-fold. Paper_evidence: WBPaper00040228
            Curator_confirmed: WBPerson712
  Associated_with: Strain: WBStrain00007668
      WBStrain00024178
      WBStrain00024189
      WBStrain00026545
      WBStrain00040642
      WBStrain00043862
      WBStrain00043865
      WBStrain00043866
      WBStrain00043867
      WBStrain00043868
  Reference: WBPaper00040228
    WBPaper00041514
    WBPaper00044152
    WBPaper00044260
    WBPaper00049163
    WBPaper00049189
    WBPaper00052880
    WBPaper00055972
    WBPaper00058832
  Species: Caenorhabditis elegans