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WormBase Tree Display for Transgene: WBTransgene00009943

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Name Class

WBTransgene00009943Public_nameeeIs611_WBPaper00040410
Summary[unc-119(+); hpl-1::eyfp]
ConstructionConstructWBCnstr00009603
Integration_methodParticle_bombardment
Construction_summaryThe hpl-1 gene was amplified by PCR from cosmid clone K08H2.6 using the primers ESMG57 GGGGTACCTCAATAAAGCGACGACAGATGTAAACA and ESMG59 CGGGATCCGCGCTCATTCCTCCTGGGATGGTTGG. The resulting 4.6 kb PCR product was cut with KpnI and BamHI and cloned into pEYFP-N1 (Clontech). The resulting plasmid was supplemented with C. elegans wild type unc-119 gene that wasobtained as an XbaI-HindIII-fragment from plasmid pMM016 and inserted into the NheI and HindIII sites.
Genetic_informationIntegrated
Associated_withStrainWBStrain00042769
ReferenceWBPaper00040410
SpeciesCaenorhabditis elegans