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WormBase Tree Display for Transgene: WBTransgene00015398

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Name Class

WBTransgene00015398Public_nameWBPaper00040844Ex1
Summary[Pdaao-1::GFP]
SynonymExpr9985_Ex
ConstructionConstructWBCnstr00014989
Construction_summaryTo generate the daao-1 promoter-GFP fusion construct, approximately 2.0 kb genomic regions upstream of the daao-1 initiation codon and the adjacent first exons were amplified by PCR. The template used was Y69A2AR . The primers used for Pdaao-1::GFP were: 5'-CTG CAG GAT CTT CCG TAT CCG-3' (forward primer) and 5'-GAG CTC GGC ATT TCT GAA AAA TAT AG-3' (reverse primer). The PCR products were cloned into a pT7Blue vector (Novagen, Madison, WI, USA), which generated pT7-Pdaao-1, and the sequences were confirmed. Subsequently, the PstI-SacI fragments containing the daao-1 promoter regions of pT7-Pdaao-1 were subcloned into pPD95.67, resulting in the Pdaao-1::GFP, reporter plasmid.
Genetic_informationExtrachromosomal
Used_forExpr_patternExpr9985
ReferenceWBPaper00040844
SpeciesCaenorhabditis elegans