WormBase Tree Display for Transgene: WBTransgene00015399
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WBTransgene00015399 | Public_name | WBPaper00040844Ex4 | |
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Summary | [Pddo-1::GFP] | ||
Synonym | Expr9986_Ex | ||
Construction | Construct | WBCnstr00014990 | |
Construction_summary | To generate the ddo-1 promoter-GFP fusion construct, approximately 3.3 kb genomic regions upstream of the ddo-1 initiation codon and the adjacent first exons were amplified by PCR. The template used was C47A10 for Pddo-1::GFP. The primers used were as follows 5'-CTG CAG CAA CAT CAT CTG ATC-3' (forward primer) and 5'-GAG CTC GTC ATT TTT GAG GAA GAA C-3' (reverse primer). The PCR products were cloned into a pT7Blue vector (Novagen, Madison, WI, USA), which generated pT7-Pddo-1, and the sequences were confirmed. Subsequently, the PstI-SacI fragments containing the ddo-1 promoter regions of pT7-Pddo-1 were subcloned into pPD95.67, resulting in the Pddo-1::GFP reporter plasmid. | ||
Genetic_information | Extrachromosomal | ||
Used_for | Expr_pattern | Expr9986 | |
Reference | WBPaper00040844 | ||
Species | Caenorhabditis elegans |