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WormBase Tree Display for Transgene: WBTransgene00015399

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Name Class

WBTransgene00015399Public_nameWBPaper00040844Ex4
Summary[Pddo-1::GFP]
SynonymExpr9986_Ex
ConstructionConstructWBCnstr00014990
Construction_summaryTo generate the ddo-1 promoter-GFP fusion construct, approximately 3.3 kb genomic regions upstream of the ddo-1 initiation codon and the adjacent first exons were amplified by PCR. The template used was C47A10 for Pddo-1::GFP. The primers used were as follows 5'-CTG CAG CAA CAT CAT CTG ATC-3' (forward primer) and 5'-GAG CTC GTC ATT TTT GAG GAA GAA C-3' (reverse primer). The PCR products were cloned into a pT7Blue vector (Novagen, Madison, WI, USA), which generated pT7-Pddo-1, and the sequences were confirmed. Subsequently, the PstI-SacI fragments containing the ddo-1 promoter regions of pT7-Pddo-1 were subcloned into pPD95.67, resulting in the Pddo-1::GFP reporter plasmid.
Genetic_informationExtrachromosomal
Used_forExpr_patternExpr9986
ReferenceWBPaper00040844
SpeciesCaenorhabditis elegans