Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Transgene: WBTransgene00015803

expand all nodes | collapse all nodes | view schema

Name Class

WBTransgene00015803Public_namewgIs108
Summary[FKH-4::TGF(11D3)::GFP::TY1::3xFLAG]
Synonym[FKH-4::GFP + unc-119(+)] [P000007_F12 protein::TY1-eGFP-3XFLAG]
ConstructionConstructWBCnstr00015379
Integration_methodParticle_bombardment
Construction_summaryInserted into fosmid WRM0617dE06 as C-terminal protein fusion. A GFP-3XFLAG tag was introduced at the C-terminus of each transcription factor's coding region in a genomic fosmid clone by recombineering (Sarov et al. 2006). An unc-119 rescue marker was introduced by recombineering into the fosmid backbone and integrated transgenic strains were made by bombardment. Amplified from WRM0615dC04.
Genetic_informationIntegrated
Used_forExpr_patternExpr10490
Expr10491
Expr10492
Expr1200007
Associated_withStrainWBStrain00033665
ReferenceWBPaper00040986
WBPaper00046510
SpeciesCaenorhabditis elegans