WormBase Tree Display for Transgene: WBTransgene00017585
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| WBTransgene00017585 | Public_name | hhIs88 | |
|---|---|---|---|
| Summary | [unc-119(+); unc-54::unc-45V480R/V484R-FLAG] | ||
| Construction | Construct | WBCnstr00016982 | |
| Coinjection_other | unc-119(+) | ||
| Integration_method | Particle_bombardment | ||
| Construction_summary | The C-terminally FLAG tagged UNC-45 was constructed by PCR amplification and cloned into pPD88.27, under the muscle specific promoter unc-54, containing the unc-119(+) marker for selection of transgenic worms. The V480R/V484R mutations were made using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). | ||
| Genetic_information | Integrated | ||
| Reference | WBPaper00041997 | ||
| Species | Caenorhabditis elegans |
