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WormBase Tree Display for Transgene: WBTransgene00018938

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Name Class

WBTransgene00018938Genetic_informationPhenotypeWBPhenotype:0000006Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
RemarkThe transgenic animals (GFP-positive) with over expressed CeCHIP were egg-laying defective (Egl) at adult stage at 20 deg C. These animals could lay few eggs but huge eggs remained inside the uterus and ultimately many eggs hatched in the uterus.Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
Caused_by_geneWBGene00000500Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
WBPhenotype:0000062Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
Remark100% of the transgenic (CHN-1 overexpressing) embryos remained unhatched, whereas 95% of the control transgenic (GFP-positive) embryos hatched. Reduced chn-1 DNA concentrations (to 25.0 ng/ul) resulted in 95% of the CHIP transgenic (GFP-positive) embryos hatching.Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
Caused_by_geneWBGene00000500Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
WBPhenotype:0000308Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
RemarkAlthough most of the transgenic F1 progeny grew with Unc and Egl phenotypes, about 30% of the F1 remained in the L1/L2 stage when grown at 25 deg C. arrested animals with 1% SDS and found that they are resistant to SDS. These data indicate that these arrested larvae are dauers .Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
PenetranceIncompletePaper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
Caused_by_geneWBGene00000500Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
WBPhenotype:0000408Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
RemarkWhen shifted from 25 to 15 deg C, these dauers did not return to a normal life cycle; CHIP transgenic animals form irreversible dauers at 25 deg C. The other 70% of the transgenic animals grew into adults.Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
PenetranceIncompletePaper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
Caused_by_geneWBGene00000500Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
WBPhenotype:0000643Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
RemarkDNA injected at a concentration of 25.0 ng/ul, where 95% of the transgenic embryos hatched, resulted in all transgenic animals (GFP-positive) being uncoordinated in locomotion (Unc) from the larval stage.Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
Caused_by_geneWBGene00000500Paper_evidenceWBPaper00006537
Curator_confirmedWBPerson712
ReferenceWBPaper00006537