WormBase Tree Display for Transgene: WBTransgene00019334
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| WBTransgene00019334 | Public_name | otEx5057 | |
|---|---|---|---|
| Summary | [GFP::vab-3 fosmid; elt-2::DsRed] | ||
| Construction | Construct | WBCnstr00018671 | |
| Coinjection_other | elt-2::DsRed | ||
| Construction_summary | The vab-3 fosmid reporter was generated by fosmid recombineering based in fosmid WRM0623aF12 (Tursun et al., 2009) using a gfp reporter fused to the N terminus of the protein and replacing the stop codon. It was injected at a concentration of 15 ng/ul, in combination with 2 ng/ml of elt-2::DsRed and 100 ng/ul OP50 gDNA (as filling DNA) to generate 3 extrachromosmal arrays (otEx5057, otEx5058, otEx5059) none of which rescue the vab-3(ot569) phenotype. | ||
| Genetic_information | Extrachromosomal | ||
| Used_for | Expr_pattern | Expr11376 | |
| Reference | WBPaper00044482 | ||
| Species | Caenorhabditis elegans |
