WormBase Tree Display for Transgene: WBTransgene00020500
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WBTransgene00020500 | Public_name | raxIs3 | |||||
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Summary | [ges-1p::lipl-4::sl2gfp] | ||||||
Synonym | lipl-4 Tg | ||||||
Construction | Construct | WBCnstr00020107 | |||||
Genetic_information | Integrated | ||||||
Phenotype | WBPhenotype:0000061 | Paper_evidence | WBPaper00046225 | ||||
Curator_confirmed | WBPerson712 | ||||||
Remark | A transgenic strain (lipl-4 Tg) that constitutively expressed lipl-4 in the intestine had 55% mean life-span increase compared with wild-type (WT) animals (Fig. 1E and table S1). Constitutive expression of LIPL-4 without the signal peptide (lipl-4 Tg no SP), which was not targeted to the lysosome,caused little extension of life span (fig. S4 and table S1), which suggests that the lysosomal activity of LIPL-4 is essential for its longevity effect. lipl-4 Tg extended span was not affected by OEA analog supplementation (Fig. 4G and table S1). | Paper_evidence | WBPaper00046225 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_gene | WBGene00019376 | Paper_evidence | WBPaper00046225 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0000135 | Paper_evidence | WBPaper00046225 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Of the nine C. elegans FABP homologs, only mRNA amounts of lbp-8 were increased in lipl-4 Tg animals, but not in the lipl-4 Tg no SP strain (Fig. 1F). acs-2 transcription wasincreased more than 15-fold in lipl-4 Tg animals. | Paper_evidence | WBPaper00046225 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_gene | WBGene00019376 | Paper_evidence | WBPaper00046225 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0000436 | Paper_evidence | WBPaper00046225 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | The partial nuclear localization of LBP-8 in the intestine, was enhanced in lipl-4 Tg animals (Fig. 2, A to G, and fig. S6, A to F). | Paper_evidence | WBPaper00046225 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_gene | WBGene00019376 | Paper_evidence | WBPaper00046225 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0000725 | Paper_evidence | WBPaper00046225 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | Among 352 metabolites detected, 71 had significantly altered abundance in lipl-4 Tg animals(table S2). Long-chain fatty acids and their derivatives are likely binding partners of FABPs(4). Thus, we focused our analysis on three C20 fatty acids - arachidonic acid, -3 arachidonic acid,and dihomo--linolenic acid and oleoylethanolamide(OEA), an N-acylethanolamine fatty acid derivative (Fig. 4A and table S2). | Paper_evidence | WBPaper00046225 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_gene | WBGene00019376 | Paper_evidence | WBPaper00046225 | ||||
Curator_confirmed | WBPerson712 | ||||||
WBPhenotype:0001739 | Paper_evidence | WBPaper00046225 | |||||
Curator_confirmed | WBPerson712 | ||||||
Remark | A transgenic strain (lipl-4 Tg) that constitutively expressed lipl-4 in the intestine had delayed age-related decline of physical activity (fig. S3A). | Paper_evidence | WBPaper00046225 | ||||
Curator_confirmed | WBPerson712 | ||||||
Caused_by_gene | WBGene00019376 | Paper_evidence | WBPaper00046225 | ||||
Curator_confirmed | WBPerson712 | ||||||
Used_for | Interactor | WBInteraction000524836 | |||||
WBInteraction000524837 | |||||||
WBInteraction000524838 | |||||||
WBInteraction000524845 | |||||||
WBInteraction000524929 | |||||||
WBInteraction000525132 | |||||||
Reference | WBPaper00046225 | ||||||
WBPaper00052979 | |||||||
WBPaper00056144 | |||||||
Species | Caenorhabditis elegans |