Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Transgene: WBTransgene00020500

expand all nodes | collapse all nodes | view schema

Name Class

WBTransgene00020500Public_nameraxIs3
Summary[ges-1p::lipl-4::sl2gfp]
Synonymlipl-4 Tg
ConstructionConstructWBCnstr00020107
Genetic_informationIntegrated
PhenotypeWBPhenotype:0000061Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
RemarkA transgenic strain (lipl-4 Tg) that constitutively expressed lipl-4 in the intestine had 55% mean life-span increase compared with wild-type (WT) animals (Fig. 1E and table S1). Constitutive expression of LIPL-4 without the signal peptide (lipl-4 Tg no SP), which was not targeted to the lysosome,caused little extension of life span (fig. S4 and table S1), which suggests that the lysosomal activity of LIPL-4 is essential for its longevity effect. lipl-4 Tg extended span was not affected by OEA analog supplementation (Fig. 4G and table S1).Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
Caused_by_geneWBGene00019376Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
WBPhenotype:0000135Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
RemarkOf the nine C. elegans FABP homologs, only mRNA amounts of lbp-8 were increased in lipl-4 Tg animals, but not in the lipl-4 Tg no SP strain (Fig. 1F). acs-2 transcription wasincreased more than 15-fold in lipl-4 Tg animals.Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
Caused_by_geneWBGene00019376Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
WBPhenotype:0000436Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
RemarkThe partial nuclear localization of LBP-8 in the intestine, was enhanced in lipl-4 Tg animals (Fig. 2, A to G, and fig. S6, A to F).Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
Caused_by_geneWBGene00019376Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
WBPhenotype:0000725Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
RemarkAmong 352 metabolites detected, 71 had significantly altered abundance in lipl-4 Tg animals(table S2). Long-chain fatty acids and their derivatives are likely binding partners of FABPs(4). Thus, we focused our analysis on three C20 fatty acids - arachidonic acid, -3 arachidonic acid,and dihomo--linolenic acid and oleoylethanolamide(OEA), an N-acylethanolamine fatty acid derivative (Fig. 4A and table S2).Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
Caused_by_geneWBGene00019376Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
WBPhenotype:0001739Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
RemarkA transgenic strain (lipl-4 Tg) that constitutively expressed lipl-4 in the intestine had delayed age-related decline of physical activity (fig. S3A).Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
Caused_by_geneWBGene00019376Paper_evidenceWBPaper00046225
Curator_confirmedWBPerson712
Used_forInteractorWBInteraction000524836
WBInteraction000524837
WBInteraction000524838
WBInteraction000524845
WBInteraction000524929
WBInteraction000525132
ReferenceWBPaper00046225
WBPaper00052979
WBPaper00056144
SpeciesCaenorhabditis elegans