WormBase Tree Display for Transgene: WBTransgene00023225
expand all nodes | collapse all nodes | view schema
| WBTransgene00023225 | Public_name | sprSi11 | |
|---|---|---|---|
| Summary | [Pmex-5::MODC PEST:GFP:H2B::cul-4 3'UTR cb-unc-119(+)] | ||
| Construction | Construct | WBCnstr00023075 | |
| Integration_method | Single_copy_insertion | ||
| Construction_summary | MosSCI protocol (Frkjaer-Jensen et al., 2008) was followed to generate the integrated lines with the adaptation described in this paper. A library of reporter constructs were injected at a concentration of 25ng/l were injected with Peft-3::mos-1 transposase (50ng/l), Pmyo-2::mCherry (2.5 ng/l), Pmyo-3::mCherry and peel-1 (10 ng/l) plasmids into the gonad of EG6699 un-coordinated strain at the young adult stage. Wild-type moving, surviving worms were screened by single-worm PCR using Pfu Ultra II to identify the integrated construct. | ||
| Genetic_information | Integrated | ||
| Map | II | ||
| Used_for | Expr_pattern | Expr12973 | |
| Associated_with | Strain | WBStrain00040514 | |
| WBStrain00046683 | |||
| WBStrain00046684 | |||
| WBStrain00046685 | |||
| Reference | WBPaper00049723 | ||
| Species | Caenorhabditis elegans |
