WormBase Tree Display for Expr_pattern: Expr10053

Expr10053 Expression_of: Gene: WBGene00021848
    Reflects_endogenous_expression_of: WBGene00021848
  Expression_data: Life_stage: WBls:0000003
      WBls:0000023
    Anatomy_term: WBbt:0003681 Uncertain
      WBbt:0004446 Uncertain
      WBbt:0004457 Uncertain
      WBbt:0004461 Uncertain
      WBbt:0004463 Uncertain
      WBbt:0004469 Uncertain
      WBbt:0004471 Uncertain
      WBbt:0005439 Partial
        Remark: Expressed in three to four pairs of neurons in the head and a pharyngeal neuron.
      WBbt:0005759 Uncertain
      WBbt:0006751 Partial
        Remark: Expressed in three to four pairs of neurons in the head and a pharyngeal neuron.
  Type: Reporter_gene
    RT_PCR
  Pattern: The nhr-239::gfp reporter was weakly expressed in three to four pairs of neurons in the head and a pharyngeal neuron in late stage embryos and all larval and adult stages. One pair of dorsal neurons express nhr-239::gfp very consistently and appear to be sensory, as do one pair of pharyngeal cells that appear to be the MC, NSM or M3 neurons.Faint fluorescence was observed in the pharynx (which may be an artefact), but did not observe nhr-239 expression in other cells at any stage. As expected from the modest expression levels observed in qRT-PCR experiments, fluorescence from the nhr-239::gfp transgene was very faint. While it is possible that the translational fusion did not recapitulate the entire nhr-239 expression pattern, this result suggests that nhr-239 is expressed only in a very limited subset of neurons.
    nhr-239 transcripts were identified by real-time qRT-PCR at very low levels (no more than 2x10-6 the level of 18 S rRNA standards) and displayed a more complicated pattern, increasing somewhat from embryos to L1, before declining progressively during later larval development.
  Picture: WBPicture0000012226
    WBPicture0000012227
  Reference: WBPaper00041200
  Transgene: WBTransgene00015436