WormBase Tree Display for Expr_pattern: Expr1035
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| Expr1035 | Expression_of | Gene | WBGene00003868 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003868 | |||
| Expression_data | Life_stage | WBls:0000007 | ||
| Anatomy_term | WBbt:0004422 | Certain | ||
| GO_term | GO:0005783 | |||
| GO:0005789 | ||||
| Subcellular_localization | Overall, OOC-3 is localized to a membrane compartment that had a dynamic distribution through the cell cycle. From fertilization until the beginning of pronuclear migration OOC-3 is uniformly enriched at the cortex of P0 in 75% of the embryos (n=36). At the beginning of pronuclear migration the cortical staining diminishes and OOC-3 is enriched around pronuclei (n=10). During rotation in P0, OOC-3 redistributes to the asters and in mitosis OOC-3 becomes enriched at the mitotic spindle. Towards the beginning of prophase of two-cell stage embryos OOC-3 localizes to a reticular structure spanning the whole cytoplasm, being enriched at the circumference of nuclei. In mitosis it again localizes to the mitotic spindle. The reticular localization can be more clearly seen in a 3-fold enlargement taken using a deconvolution microscope. ER localization was confirmed by staining C. elegans embryos with an antibody to HDEL, a sequence responsible for retention of proteins in the ER. OOC-3 is slightly enriched at the cell-cell boundary between AB and P1 in 50% of the embryos examined (n=45). | |||
| Type | Antibody | A polyclonal C-terminal anti-peptide antibody (OOC3Ct2) against a peptide spanning aa 432-448. | ||
| Picture | WBPicture0000008046 | |||
| WBPicture0000008047 | ||||
| Remark | No detailed cellular pattern description later than 2 cell stage embryo. | |||
| Reference | WBPaper00004183 | |||
| Antibody_info | WBAntibody00000283 |
