WormBase Tree Display for Expr_pattern: Expr1042
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Expr1042 | Expression_of | Gene | WBGene00003219 | |
---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00003219 | |||
Expression_data | Life_stage | WBls:0000005 | ||
Anatomy_term | WBbt:0004421 | Certain | ||
WBbt:0004804 | Certain | |||
WBbt:0006873 | Certain | |||
WBbt:0006875 | Certain | |||
GO_term | GO:0005887 | |||
GO:0071944 | ||||
Subcellular_localization | MES-1 staining was present adjacent to and sometimes overlapped with the cell cortex (marked by actin staining). | |||
Type | Antibody | Rat antisera were raised against a 6His-tagged fusion protein containing the 475 amino acid intracellular domain of MES-1 and were then affinity purified. | ||
Pattern | MES-1 is detected as a thin crescent, initially in four-cell embryos between P2 and EMS and then in eight- to 12-cell embryos between P3 and E. MES-1 persists through the formation of P4 at the 24-cell stage. After P4 is generated, MES-1 begins to fade and is usually not detectable beyond the 30-40-cell stage. The crescent shape of MES-1 staining outlined a portion of the contact area between the germline and the gut cell; the tips of the crescent point dorsally, towards the interior of the embryo. From late prophase through anaphase, the more ventral centrosome was closely juxtaposed to the MES-1 crescent, with the two appearing to almost touch. By telophase, MES-1 and the centrosome had separated. MES-1 staining is localized in the germline cell starting with P2 (by P2 cell dissociation assay). Co-staining of MES-1 and actin shows the cytoplasmic domain of MES-1 to be on the P-cell side of the zone of germline-gut contact. | |||
Picture | WBPicture0000008063 | |||
WBPicture0000008064 | ||||
WBPicture0000008065 | ||||
WBPicture0000008066 | ||||
Remark | In this paper authors refer to EMS as a gut cell, though it generates more than just gut. | |||
Reference | WBPaper00004323 | |||
Antibody_info | WBAntibody00000304 |