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WormBase Tree Display for Expr_pattern: Expr1042

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Name Class

Expr1042Expression_ofGeneWBGene00003219
Reflects_endogenous_expression_ofWBGene00003219
Expression_dataLife_stageWBls:0000005
Anatomy_termWBbt:0004421Certain
WBbt:0004804Certain
WBbt:0006873Certain
WBbt:0006875Certain
GO_termGO:0005887
GO:0071944
Subcellular_localizationMES-1 staining was present adjacent to and sometimes overlapped with the cell cortex (marked by actin staining).
TypeAntibodyRat antisera were raised against a 6His-tagged fusion protein containing the 475 amino acid intracellular domain of MES-1 and were then affinity purified.
PatternMES-1 is detected as a thin crescent, initially in four-cell embryos between P2 and EMS and then in eight- to 12-cell embryos between P3 and E. MES-1 persists through the formation of P4 at the 24-cell stage. After P4 is generated, MES-1 begins to fade and is usually not detectable beyond the 30-40-cell stage. The crescent shape of MES-1 staining outlined a portion of the contact area between the germline and the gut cell; the tips of the crescent point dorsally, towards the interior of the embryo. From late prophase through anaphase, the more ventral centrosome was closely juxtaposed to the MES-1 crescent, with the two appearing to almost touch. By telophase, MES-1 and the centrosome had separated. MES-1 staining is localized in the germline cell starting with P2 (by P2 cell dissociation assay). Co-staining of MES-1 and actin shows the cytoplasmic domain of MES-1 to be on the P-cell side of the zone of germline-gut contact.
PictureWBPicture0000008063
WBPicture0000008064
WBPicture0000008065
WBPicture0000008066
RemarkIn this paper authors refer to EMS as a gut cell, though it generates more than just gut.
ReferenceWBPaper00004323
Antibody_infoWBAntibody00000304