Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Expr_pattern: Expr1047

expand all nodes | collapse all nodes | view schema

Name Class

Expr1047Expression_ofGeneWBGene00000908
Reflects_endogenous_expression_ofWBGene00000908
Expression_dataLife_stageWBls:0000002
Anatomy_termWBbt:0004145Certain
WBbt:0004147Certain
WBbt:0004149Certain
WBbt:0004151Certain
WBbt:0004153Certain
WBbt:0004155Certain
WBbt:0004409Certain
WBbt:0004410Certain
WBbt:0004448Certain
WBbt:0004477Certain
WBbt:0004489Certain
WBbt:0004506Certain
WBbt:0004520Certain
WBbt:0004574Certain
WBbt:0004577Certain
WBbt:0005175Certain
WBbt:0005300Certain
WBbt:0005319Certain
WBbt:0005451Certain
WBbt:0005733Certain
WBbt:0005772Certain
WBbt:0005813Certain
WBbt:0006748Certain
WBbt:0006751Partial
WBbt:0006759Partial
WBbt:0006760Certain
WBbt:0006762Certain
WBbt:0006763Certain
WBbt:0006764Certain
WBbt:0006765Certain
WBbt:0006766Certain
WBbt:0006767Certain
WBbt:0006768Certain
WBbt:0006770Certain
WBbt:0006771Certain
WBbt:0006772Certain
WBbt:0006773Certain
WBbt:0006774Certain
WBbt:0006775Certain
WBbt:0006776Certain
WBbt:0006777Certain
WBbt:0006778Certain
WBbt:0006779Certain
WBbt:0006983Certain
WBbt:0006984Certain
WBbt:0006987Certain
WBbt:0006988Certain
WBbt:0006989Certain
WBbt:0006990Certain
WBbt:0006991Certain
WBbt:0007252Certain
WBbt:0007253Certain
WBbt:0007254Certain
WBbt:0007255Certain
WBbt:0007256Certain
WBbt:0007260Certain
WBbt:0007264Certain
WBbt:0007265Certain
WBbt:0007266Certain
WBbt:0008112Certain
WBbt:0008117Certain
WBbt:0008121Certain
WBbt:0008125Certain
WBbt:0008129Certain
WBbt:0008133Certain
GO_termGO:0005634
Subcellular_localizationDAF-12::GFP localized primarily to the nucleus, except during mitosis, when expression became diffuse.
TypeReporter_gene
PatternDAF-12::GFP was expressed widely in most cells including tissues modified for dauer formation or by stage. It was expressed in phenotypically affected target tissues (e.g., epidermis, vulva, somatic gonad, intestine, pharynx, sex myoblasts), as well as other tissues with no known phenotype (e.g., nervous system, body wall muscle). Expression was seen from embryo to adult, but was most elevated and widespread during L2. Epidermis: In seam cells and hypodermis, DAF-12::GFP expression was first seen at the 3-fold stage of embryogenesis, increased by late L1, peaked during L2, diminished by late L3, and was low or off in L4 and young adults. Expression was also seen in the ventral epidermal L1 P ectoblasts, L2 vulval precursors, and their L3 descendants. Expression continued during L4 vulval morphogenesis and persisted occasionally in the mature adult vulva at reduced levels. Somatic Gonad: Faint expression was seen as early as L1 in Z1 and Z4 somatic gonadal precursors. By L2, their descendants, the somatic gonadoblasts, including the migratory distal-tip cell, strongly expressed DAF-12::GFP. Expression continued in somatic gonadoblast descendants and distal-tip cells in L3 and early L4. In the adult, expression was robust in the mature spermatheca and uterus. Intestine: Expression in intestinal nuclei was diffuse during the larval stages, but became somewhat stronger in the adult. Nervous System: Only a handful of head and tail neurons expressed GFP early in L1. By mid-L2, DAF-12::GFP was expressed strongly throughout the nervous system, including the ventral cord and peripheral neuroblasts. Expression continued in many neurons in the adult, albeit at reduced levels. Musculature: Expression in body wall muscles became visible by late L1 and L2. Expression continued at later larval stages and in the adult at reduced levels. Expression in pharyngeal muscle was strong by L2 and downregulated by adult. DAF-12::GFP was also expressed in the L1 M-mesoblast, and its derivatives, including post-embryonic body wall muscles, sex myoblasts and their descendants. Dauer formation: DAF-12::GFP was downregulated in dauer larvae in all tissues, but perdured in the somatic gonad and occasional neurons. Upon recovery from dauer diapause, DAF-12::GFP was expressed weakly in most tissues.
PictureWBPicture0000008399
RemarkLineage expression: M lineage.
The transgene rescued the Daf-d phenotype of rh61rh411 animals, suggesting that daf-12::GFP is functional. Several integrated transgenic lines were made and all gave a similar pattern of expression.
ReferenceWBPaper00004181
TransgeneWBTransgene00027311