WormBase Tree Display for Expr_pattern: Expr11020

Expr11020 Expression_of: Gene: WBGene00003915
    Reflects_endogenous_expression_of: WBGene00003915
  Expression_data: Life_stage: WBls:0000002
    Anatomy_term (11)
    GO_term: GO:0005886
      GO:0005737
      GO:0045177
  Subcellular_localization: PAN-1FL::GFP exhibited a punctate localization in expressing cells. In the vulva and somatic gonad epithelia (spermatheca and uterus), these punctae were concentrated on the apical (luminal) surface of these cells. During embryonic pharyngeal development, PAN-1FL::GFP was found in the apical pharyngeal epithelium, as indicated by co-staining with the monoclonal antibody MH27 which recognizes the adherens junctions of epithelial cells. The localization in apical regions of epithelial cells is consistent with membrane association of the PAN-1B isoform. Additionally, immunolocalization data for PAN-1 indicate membrane association in intestinal cells. Cytoplasmic expression of GFP is also observed (likely corresponding to PAN-1A or PAN-1C); especially in the pharynx but also in other cell types. However, apparent cytoplasmic expression was not consistently observed indicating that some cell types may only express cytoplasmic isoforms of PAN-1 at certain developmental times.
  Type: Reporter_gene
  Pattern: Examination of transgenic animals bearing the transcriptional fusion revealed that pan- 1prom::GFP is expressed in most somatic tissues. Expression was observed in all developmental stages from embryos undergoing morphogenesis to gravid adults. pan- 1prom::GFP exhibited strong expression in body wall muscle, vulva, somatic gonad and pharynx. Expression was also observed in the nerve ring, hypodermis, and rectal epithelia. Expression in the intestine was typically not observed or very weak. With respect to spatial expression, the pattern of pan-1FL::GFP was the same as pan-1prom::GFP with some notable exceptions. First, PAN- 1FL::GFP exhibited differences in expression intensity depending on the developmental point within the intermolt period. PAN-1FL::GFP was substantially qualitatively brighter at the mid to late intervals of the intermolt. Early in the intermolt bright expression of PAN- 1FL::GFP was typically only observed in the pharynx. Expression was also weak in the adult with the pharynx being the predominant expressing tissue. The other difference with respect to expression is that PAN-1FL::GFP was observed in the intestine, especially when expression was bright towards the end of the molt.
  Picture: WBPicture0000013204
  Reference: WBPaper00042412
  Transgene: WBTransgene00018475