WormBase Tree Display for Expr_pattern: Expr11197
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| Expr11197 | Expression_of | Gene | WBGene00006525 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006525 | ||
| Expression_data | GO_term | GO:0097730 | |
| GO:0044441 | |||
| Subcellular_localization | TAX-2::GFP expressed under an AWB-specific promoter (str-1p::tax-2::gfp) was localized to a proximal region of each of the AWB neuron ciliary branches and excluded from the distal regions. The region of TAX-2 localization was distal to the basal body/transition fiber zone marked by CHE-13::TagRFP IFT protein accumulation, as well as the transition zone marked by localization of an MKSR-2::TagRFP fusion protein. The region of TAX-2::GFP localization was instead reminiscent of the Inversin compartment observed in several cilia types in vertebrates. As in mammalian cells, the Inversin homolog NPHP-2 is localized to the middle segment in C. elegans sensory cilia and shown to play a role in placement of the transition zone. The authors found that localization of TAX-2::GFP in AWB cilia overlapped almost fully with that of NPHP-2::mCherry. TAX-2::GFP expression was not observed in AWB axons. | ||
| When expressed under an ASK-specific promoter (srbc-66), TAX-2 was localized to the middle segment, distal to the basal body/transition fiber region. Therefore TAX-2 is localized to the middle segments of both AWB and ASK cilia. | |||
| Type | Reporter_gene | [srbc-66p::tax-2::gfp] translational fusion. srbc-66p::tax-2::gfp was generated by fusing tax-2 cDNA in frame to gfp in an expression vector containing 2.1 kb of srbc-66 upstream regulatory sequences (Kim et al., 2009). | |
| Reference | WBPaper00043992 | ||
| Transgene | WBTransgene00030311 |
