WormBase Tree Display for Expr_pattern: Expr11229
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| Expr11229 | Expression_of | Gene | WBGene00194892 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00194892 | |||
| Expression_data | Anatomy_term | WBbt:0006796 | Certain | |
| GO_term | GO:0005694 | |||
| GO:0000785 | ||||
| Subcellular_localization | Immunofluorescence experiments using an antibody against the DSB-2 protein showed that DSB-2 localizes to chromatin in germ cell nuclei from meiotic entry to mid-pachytene. DSB-2 staining is first detected in the transition zone (TZ; corresponds to leptotene and zygotene stages, when pairing and SC assembly occur), and is strongest overall in early pachytene, where it localizes to chromatin in an uneven pattern, showing a few bright patches per nucleus as well as fainter stretches/foci associated with most of the chromatin. Towards mid-pachytene, the bright patches diminish and the chromatin signal fades and then disappears from most nuclei. However, in a subset of nuclei in the mid/late pachytene region, DSB-2 staining becomes brighter, with bright stretches/foci along most of the chromatin; a few of these 'outlier' brightly-staining nuclei are present in later pachytene and likely represent nuclei destined for apoptosis. | |||
| Type | Antibody | |||
| Picture | WBPicture0000013313 | |||
| Reference | WBPaper00044072 | |||
| Antibody_info | WBAntibody00002458 |
