WormBase Tree Display for Expr_pattern: Expr11268
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| Expr11268 | Expression_of | Gene | WBGene00008570 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00008570 | ||
| Expression_data | Anatomy_term (11) | ||
| Type | Reporter_gene | ||
| Pattern | Four constructs were made where GFP was fused to either initiation site or stop codon in order to determine if the splice variants are differentially expressed. Expression of the transformation vectors was driven under 1,951 bp of the KCNL-2 endogenous promoter. Confocal fluorescent imaging of transgenic lines expressing these constructs in the N2 background showed a neuronal expression pattern. expression of KCNL-2 when tagged at the latter stop codon (pkcnl-2kcnl-2(taa2)::gfp) was detected in head neurons, the nerve ring (NR), motor neurons of the ventral nerve cord (VNC), the dorsal cord (DC) and tail ganglia. The pkcnl-2kcnl-2(taa2)::gfp construct was expressed in many neuronal processes innervating the vulva while pkcnl-2gfp::(atg2)kcnl-2 shows expression in the VC4 and VC5 neurons of the egg-laying apparatus in addition to other neuronal processes innervating the vulva. | ||
| The authors made two additional constructs to drive GFP expression under the KCNL-2 promoter to aid in determining the complete expression profile of KCNL-2 that may be masked in transgenic lines that express GFP-tagged KCNL-2.The first construct, pkcnl-2(atg1) gfp, encompassed the coding sequence of GFP which was expressed downstream of the promoter of KCNL-2 (1,951 bp upstream of atg1). The second construct, pkcnl-2(atg2) gfp, expressed GFP at the second initiation site where the coding sequences for kcnl-2-b and -c were deleted. pkcnl-2(atg1) gfp showed a neuronal expression profile with additional GFP-expression in the vulval muscles. pkcnl-2(atg2) gfp showed a strict neuronal expression profile that complemented the expression profile of the pkcnl-2kcnl-2::gfp constructs. pkcnl-2(atg2) gfp readily labels the VC4 and VC5 neurons and displays a highly innervated vulva, a feature that is lacking in the expression profile of pkcnl-2(atg1) gfp. Ultimately, since both pkcnl-2(atg1) gfp and pkcnl-2(atg2) gfp are expressed in different neurons, this suggests that the various isoforms of KCNL-2 may have differing neuronal expression profiles. | |||
| Picture | WBPicture0000013480 | ||
| WBPicture0000013481 | |||
| Reference | WBPaper00044193 | ||
| Transgene | WBTransgene00019125 | ||
| WBTransgene00019126 | |||
| WBTransgene00019127 | |||
| WBTransgene00019128 |
