WormBase Tree Display for Expr_pattern: Expr11400
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| Expr11400 | Expression_of | Gene | WBGene00003760 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003760 | |||
| Expression_data | Life_stage | WBls:0000023 | ||
| Anatomy_term | WBbt:0003958 | Certain | ||
| WBbt:0003960 | Certain | |||
| WBbt:0005739 | Certain | |||
| WBbt:0005829 | Certain | |||
| Type | Reporter_gene | [pnlp-22::GFP] transcriptional fusion and [Pnlp-22::nlp-22:gfp; Pmyo-2::mCherry; unc-119(+)] translational fusion. The transcriptional reporter for nlp-22 (Pnlp-22:Intron:gfp) was made by fusing the promoter of nlp-22 (-450 to +3) to the coding sequence of GFP, amplified from pPD95.75 and replacing one of the synthetic introns of gfp with the intron of nlp-22( +85 to +481). | ||
| RT_PCR | ||||
| Pattern | NLP-22 is selectively expressed, in a single-neuron pair, the RIAs. NLP-22 is predicted to be secreted. When GFP was fused to the C-terminus of the NLP-22 preproprotein, green fluorescence was observed in several neurons in the head as well as in the ventral nerve cord, supporting the notion that NLP-22 is a secreted peptide. | |||
| mRNA abundance of the gene nlp-22 was higher in the L4 stage than in lethargus. Using qRT-PCR, we confirmed that nlp-22 mRNA expression cycles with a constant phase relationship to lethargus. nlp-22 mRNA peaked midway through the active larval stages, decreased during each lethargus stage, and was absent in adults. | ||||
| Reference | WBPaper00044546 | |||
| Transgene | WBTransgene00019305 |
