WormBase Tree Display for Expr_pattern: Expr11565
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| Expr11565 | Expression_of | Gene | WBGene00000939 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000939 | |||
| Expression_data | Anatomy_term | WBbt:0005784 | Certain | |
| WBbt:0008378 | Certain | |||
| GO_term | GO:0005634 | |||
| GO:0005886 | ||||
| GO:0005737 | ||||
| GO:0043186 | ||||
| GO:0048471 | ||||
| Subcellular_localization | Throughout the germline, DCR-1 was detected at low levels in uniformly-distributed small granules, more diffusely throughout the cytoplasm, and in some P granules. In the meiotic pachytene region, DCR-1 was also concentrated in distinct foci inside the nucleus that were sometimes adjacent to puncta stained by mAb414, an antibody that recognizes several nucleoporin proteins; however, the degree of co-localization was not high, with a Pearson's coefficient of 0.26. Around pachytene nuclei, DCR-1 occasionally co-localized with GLH-1 in P granules but did not have a high degree of co-localization with GLH-1; here the Pearson's coefficient was 0.36. While the distribution of DCR-1 in germ cell nuclei included puncta on the inner face of nuclear pores, GLH-1 was almost exclusively perinuclear and highly enriched in P granules on the cytoplasmic side of nuclear pores. The Pearson's coefficient of 0.82 suggests a high degree of co-localization between GLH-1 and nuclear pores. Thus, although DCR-1 and GLH-1 do not appear to have a high degree of co-localization, the GLH-1/DCR-1 biochemical interaction may reflect a transient interaction at or near nuclear pores. While GLH-1 is highly enriched at nuclear pores, DCR-1 seems likely to traffic through the nuclear pores as it is detected both inside nuclei and in the cytoplasm. In maturing wild type oocytes, DCR-1 was also detected throughout the cytoplasm and nucleus, sometimes slightly enriched at plasma membranes. | |||
| Type | Antibody | |||
| Pattern | By immunocytochemistry, DCR-1 was present throughout both the C. elegans germline and the somatic tissue (most easily seen in the extruded intestine) in wild-type worms. | |||
| Remark | Temporal description | |||
| Reference | WBPaper00037884 | |||
| Antibody_info | WBAntibody00002257 |
