WormBase Tree Display for Expr_pattern: Expr12872

Expr12872 Expression_of: Gene: WBGene00003031
    Reflects_endogenous_expression_of: WBGene00003031
  Expression_data: Life_stage: WBls:0000028 Anatomy_term: WBbt:0005753
      WBls:0000036 Anatomy_term: WBbt:0005753
    Anatomy_term: WBbt:0005733 Certain
      WBbt:0005753 Certain
        Life_stage: WBls:0000028
          WBls:0000036
    GO_term: GO:0005634
      GO:0030424
      GO:0005737
      GO:0043025
  Subcellular_localization: The translational fusion also showed strong constitutive expression in the cell bodies and axons of the bilateral motor interneurons AVB, but we do not know the significance of this expression. The LIN-46:GFP fusion protein localized to both cytoplasm and nucleus, but mostly in the non-nucleolar part of the nucleus.
  Type: Reporter_gene
  Pattern: Green fluorescent protein (GFP) reporter constructs indicated that lin-46 is expressed in hypodermal cells, particularly the lateral and ventral hypodermis, in brief periods during larval development. Animals bearing a transcriptional fusion showed intense fluorescence in lateral hypodermal cells around the time of each molt, especially during the L2- and L4-lethargus. In L2 animals, not all seam cells fluoresced simultaneously, and often only a cluster of an even number of cells fluoresced in a given animal, suggesting the expression of the fusion is related to seam cell division, which occurs somewhat asynchronously during larval development (Sulston and Horvitz, 1977). A lin-46 translational fusion reporter showed a similar expression pattern, although it was less intense. The translational fusion also showed strong constitutive expression in the cell bodies and axons of the bilateral motor interneurons AVB, but we do not know the significance of this expression. The LIN-46:GFP fusion protein localized to both cytoplasm and nucleus, but mostly in the non-nucleolar part of the nucleus.
    Green fluorescent protein (GFP) reporter constructs indicated that lin-46 is expressed in hypodermal cells, particularly the lateral and ventral hypodermis, in brief periods during larval development. Animals bearing a transcriptional fusion showed intense fluorescence in lateral hypodermal cells around the time of each molt, especially during the L2- and L4-lethargus. In L2 animals, not all seam cells fluoresced simultaneously, and often only a cluster of an even number of cells fluoresced in a given animal, suggesting the expression of the fusion is related to seam cell division, which occurs somewhat asynchronously during larval development (Sulston and Horvitz, 1977).
  Reference: WBPaper00013416
  Transgene: WBTransgene00023111