WormBase Tree Display for Expr_pattern: Expr13046
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| Expr13046 | Expression_of | Gene | WBGene00003777 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003777 | |||
| Expression_data | Anatomy_term | WBbt:0007846 | Certain | |
| WBbt:0008594 | Certain | |||
| GO_term | GO:0005913 | |||
| GO:1904269 | ||||
| Subcellular_localization | Before or during early stages of ventral enclosure, NMY-2:GFP localizes to cell boundaries and as foci that are scattered around the embryo. Just prior to pocket closure, the foci form a ring-like pattern around the ventral pocket, which appears to enrich as the pocket closes. Some of the foci in the ventral epidermal cells overlap with adhesion junctions, visualized by staining fixed embryos with MH27 antibodies that detect the adherens junction marker AJM-1, while other foci localize along the junction-free edges of the cells. Myosin also localizes near the surface of cells in the ventral pocket. Myosin appears to assemble into an interconnected network that spans multiple epidermal cells. Interestingly, the foci in the epidermal cells align with ANI-1 (C. elegans anillin)-positive neuroblasts found beneath the epidermal cells, while other foci appeared to be in the neuroblasts. The localization of myosin as foci in both the epidermal cells and neuroblasts suggests that myosin may be associated with contractile events in both cell types during ventral enclosure. The myosin foci in both the epidermal and neuroblast cells are highly dynamic during ventral enclosure and display intercellular patterns - as a ring around the pocket, and as star-like networks in the neuroblasts. | |||
| Type | Genome_editing | |||
| Reporter_gene | ||||
| Reference | WBPaper00049309 | |||
| Variation | WBVar02147463 |
