WormBase Tree Display for Expr_pattern: Expr1431
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| Expr1431 | Expression_of | Gene | WBGene00004013 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004013 | ||
| Expression_data | Life_stage | WBls:0000002 | |
| Anatomy_term (12) | |||
| GO_term | GO:0005634 | ||
| Subcellular_localization | nuclear | ||
| Type | Antibody | Affinity-purified rabbit polyclonal PHA-4 antibody. | |
| Pattern | In six comma-stage embryos, an average of 80.5 ( 2.5 s.d.) PHA-4-staining pharyngeal nuclei were counted by confocal microscopy (maximum count = 85). Analysis of later stage animals shows clearly that all six cells of the pharyngeal-intestinal valve contain PHA-4 protein. Thus the total number of potentially staining nuclei (pharynx + valve) should be equal to 86. all cells of the pharyngeal primordium (+ valve) contain the nuclear factor PHA-4. The same embryos also show nuclear PHA-4 in 6-8 rectal cells, including the two rectal valve cells and the three rectal epithelial cells. At the lima bean and comma stages of embryogenesis, a low but significant level of PHA-4 can be detected in the gut. pha-4 gene is expressed in pharyngeal precursor cells well before the formation of the pharynx primordium. PHA-4 can be detected immunologically in most but not all nuclei of the larval and adult pharynx. PHA-4 is detected in nuclei of all epithelial cells, muscle cells, marginal cells, gland cells and pharyngeal intestinal valve cells, but is detected only in about 8 of 19 neuronal nuclei. PHA-4 is also detected in one (comma stage) to several (pretzel stage and later) head cells outside of the pharynx. PHA-4 can also be detected in nuclei of the developing somatic gonad, including the distal tip cell and ventral uterine cells. | ||
| Picture | WBPicture0000007976 | ||
| Reference | WBPaper00003068 | ||
| Antibody_info | WBAntibody00000175 |
