WormBase Tree Display for Expr_pattern: Expr14590
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| Expr14590 | Expression_of | Gene | WBGene00001368 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001368 | ||||
| Expression_data | Life_stage | WBls:0000024 | Anatomy_term | WBbt:0005739 | |
| WBbt:0005741 | |||||
| WBbt:0005775 | |||||
| WBbt:0005829 | |||||
| WBls:0000016 | Anatomy_term | WBbt:0005775 | |||
| WBls:0000063 | Anatomy_term (194) | ||||
| Anatomy_term (197) | |||||
| Type | Genome_editing | ||||
| Reporter_gene | |||||
| Pattern | Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell. | ||||
| Picture | WBPicture0000014930 | ||||
| Reference | WBPaper00058767 | ||||
| Variation | WBVar02150076 |
