WormBase Tree Display for Expr_pattern: Expr15073
expand all nodes | collapse all nodes | view schema
| Expr15073 | Expression_of | Gene | WBGene00000301 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000301 | ||||
| Expression_data | Anatomy_term | WBbt:0005784 | Certain | ||
| WBbt:0008629 | Partial | ||||
| Remark | CAV-1::GFP was not clearly detected in the distal gonad until the bend region of the gonad arm. | ||||
| Type | Genome_editing | ||||
| Reporter_gene | |||||
| Pattern | Imaging endogenous CAV-1::GFP revealed similar patterns as previously reported in the germ line (Sato et al. 2006). However, unlike the pie-1 driven exogenous line, endogenous CAV-1::GFP was not clearly detected in the distal gonad until the bend region of the gonad arm, where it localized to the plasma membrane, and vesicle-shaped structures. Oocytes had slightly higher expression showing enrichment at plasma membrane localization but faint cytoplasmic signal, and no obvious enrichment to vesicles. After ovulation, CAV-1::GFP remained on the plasma membrane during meiosis I and was no longer detected by meiosis II. In 5/5 anaphase I embryos, CAV-1::GFP was not significantly localized to cortical granules at a time when separase could be observed on vesicles. Overall, endogenous CAV-1::GFP signal is less intense in the germline than exogenous pie-1-driven lines. However, endogenous CAV-1::GFP signal became increasingly intense at the plasma membrane sometime after the 16-cell embryo stage. The membrane signal remains intense through later stages of embryonic development. Finally, adult somatic expression appeared to follow previously observed patterns (Parker et al. 2007). | ||||
| Picture | WBPicture0000014982 | ||||
| Reference | WBPaper00060329 | ||||
| Variation | WBVar02153186 |
