WormBase Tree Display for Expr_pattern: Expr15171
expand all nodes | collapse all nodes | view schema
| Expr15171 | Expression_of | Gene | WBGene00019975 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00019975 | ||
| Expression_data | Anatomy_term (17) | ||
| Type | Reporter_gene | ||
| Pattern | We generated a fosmid-based reporter transgene for flp-34 and found consistent fluorescence in several head and tail neurons, as well as in the vulval region. The flp-34 reporter transgene was expressed in all serotonergic neurons (NSM, HSN and ADF (Horvitz et al., 1982)), validated by colocalization of the GFP signal with the expression of a red reporter transgene for tryptophan hydroxylase 1 (tph-1p::DsRed2) that encodes a serotonin biosynthesis enzyme (Sze et al., 2002). The flp-34 transgene was also expressed in the ASG sensory neurons as confirmed by colocalization with an ASG specific reporter (nlp-44p::mCherry), in the tail PHA sensory neurons as confirmed by DiI staining, and in the PLN and ALN neurons as identified by their morphology and position. | ||
| Reference | WBPaper00059878 | ||
| Transgene | WBTransgene00027170 |
