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WormBase Tree Display for Expr_pattern: Expr15986

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Name Class

Expr15986Expression_ofGeneWBGene00000900
Reflects_endogenous_expression_ofWBGene00000900
Expression_dataAnatomy_termWBbt:0003681Certain
WBbt:0005733Certain
WBbt:0005772Certain
WBbt:0006748Certain
GO_termGO:0016323
GO:0097708
TypeGenome_editing
Reporter_gene
PatternWe found that DAF-4::GFP::3xFLAG is expressed in multiple tissues, including the pharynx, hypodermis, intestine, and developing vulva. Within each cell, DAF-4::GFP::3xFLAG is primarily localized to the cell surface. We also detected some GFP signal in intracellular vesicles in hypodermal cells. Notably, in both the intestine and the vulva, 2 types of polarized epithelial cells, DAF-4::GFP::3xFLAG is localized to the basolateral membrane but absent on the apical membrane. The basolateral localization of DAF-4::GFP::3xFLAG in the intestinal cells has also been previously reported by Gleason and colleagues using a functional, intestinally expressed DAF-4::GFP.
ReferenceWBPaper00059222
VariationWBVar02157765