WormBase Tree Display for Expr_pattern: Expr1604
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| Expr1604 | Expression_of | Gene | WBGene00001820 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001820 | ||||
| Expression_data | Life_stage | WBls:0000018 | |||
| WBls:0000016 | |||||
| WBls:0000017 | |||||
| WBls:0000010 | |||||
| WBls:0000013 | |||||
| Anatomy_term | WBbt:0008594 | Partial | |||
| Remark | HAM-1 is restricted to the posterior half of the HSN/PHB neuroblast. | ||||
| GO_term | GO:0005737 | ||||
| GO:0071944 | |||||
| Subcellular_localization | HAM-1 expressed in cells at metaphase. Chromosomes are condensed and HAM-1 is distributed to one side of the cell. HAM-1 expressed in cells at telophase. Two nuclear envelopes have reformed and HAM-1 is distributed to the side of the cell that will be inherited with the posterior nucleus. HAM-1 staining also appears as rings that surround cells. | ||||
| HAM-1 often localizes to the periphery of cells in crescent shaped patterns that are restricted to one side of dividing cells. | |||||
| Type | Antibody | A mouse polyclonal antiserum to a His-tagged HAM-1 fusion protein expressed in E. coli was generated and used to stain whole mounts of wild-type embryos and larvae. | |||
| Pattern | HAM-1 is first expressed at the onset of gastrulation, approximately 100 minutes after cleavage of the zygote. At this time, HAM-1 often localizes to the periphery of cells in crescent shaped patterns that are restricted to one side of dividing cells. HAM-1 expression persists until the 1.5 fold stage of embryonic development, approximately 430 minutes after cleavage of the zygote. No HAM-1 expression can be detected later during embryonic or larval hermaphrodite development. | ||||
| HAM-1 protein is expressed in many mitotic and postmitotic cells. To determine whether HAM-1 is expressed in the lineage that produces the HSN and PHB neurons, embryos were stained at 260-300 minutes after cleavage of the zygote. HAM-1 is restricted to the posterior half of the HSN/PHB neuroblast. After division of the HSN/PHB neuroblast, HAM-1 ring staining can occasionally be seen in cells that, by position, could be the HSN/PHB precursor. This staining, however, is usually not detectable suggesting that HAM-1 is degraded rapidly after division of the HSN/PHB neuroblast. | |||||
| Picture | WBPicture0000007949 | ||||
| Reference | WBPaper00002607 | ||||
| Antibody_info | WBAntibody00000132 |
