WormBase Tree Display for Expr_pattern: Expr16255
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| Expr16255 | Expression_of | Gene | WBGene00000437 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000437 | ||
| Expression_data | Life_stage | WBls:0000003 | |
| Type | Reporter_gene | ||
| Pattern | For ceh-13, we found that the fosmid-based reporter wgIs756[ceh-13::EGFP] was only expressed in the embryos and no significant GFP signal was detected in larvae or adult animals. However, Tihanyi et al. reported that a transcriptional reporter ceh-13p::GFP, which contains 8.1 kb sequence upstream of the start codon, was expressed in larval stages and in many cell lineages during development. To investigate this discrepancy, we generated a GFP knock-in at the ceh-13 locus through CRISPR/Cas9-mediated gene editing and found that the expression of endogenous ceh-13::GFP was only observed in the early embryos, similar to wgIs756[ceh-13::EGFP]. Although we occasionally observed very weak ceh-13::GFPexpression in some presumably ventral nerve cord motor neurons in adults, we werenot able to consistently identify the neurons that showed the weak GFP signal. So, we concluded that the expression of ceh-13 is mostly restricted to the embryonic stages, and its expression in terminally differentiated cells is rather weak and may be below our detection limit. | ||
| Reference | WBPaper00062521 | ||
| Transgene | WBTransgene00033341 |
