WormBase Tree Display for Expr_pattern: Expr16267
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| Expr16267 | Expression_of | Gene | WBGene00017578 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00017578 | |||
| Expression_data | Anatomy_term | WBbt:0005733 | Certain | |
| WBbt:0005772 | Certain | |||
| Type | Reporter_gene | |||
| Pattern | We generated a more complete drl-1p::GFP reporter by fusing GFP coding sequence to a 5.1 kb genomic fragment that includes the upstream intergenic region and intron 1 and co-injected it with pharynx marker myo-2p::tdTomato. drl-1p::GFP was expressed in the epidermis and intestine consistent with previous mRNA enrichment studies (Pauli et al. 2006; Spencer et al. 2011; Blazie et al. 2017; Han et al. 2017). These results suggest that the upstream intergenic region drives expression in the intestine and that intron 1 also permits expression in the epidermis. A prior study failed to observe intestinal expression with a drl-1 transcriptional reporter (Chamoli et al. 2014); however, this former construct lacked the intergenic region upstream from exon 1 that drives intestinal expression. | |||
| Reference | WBPaper00062171 | |||
| Transgene | WBTransgene00033563 |
