WormBase Tree Display for Expr_pattern: Expr1626
expand all nodes | collapse all nodes | view schema
| Expr1626 | Expression_of | Gene | WBGene00003497 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003497 | |||
| Expression_data | Life_stage | WBls:0000041 | ||
| WBls:0000023 | ||||
| WBls:0000020 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005237 | Certain | |||
| WBbt:0005733 | Certain | |||
| WBbt:0005753 | Certain | |||
| WBbt:0005813 | Certain | |||
| GO_term | GO:0005737 | |||
| GO:0005882 | ||||
| Subcellular_localization | The observed pattern of MUP-4 is reminiscent of the pattern of the MH4 mAb, which recognizes IFs of hemidesmosomes. The relationship between MUP-4 and IF localization was further examined by overlay of confocal images of double-labeled animals. This analysis demonstrated that their patterns largely coincide. In addition to the annular localization, MUP-4 and IF proteins are sometimes seen diffusely throughout the hypodermal syncytial cell cytoplasm, possibly reflecting protein not yet recruited to the regions overlying muscle. MUP-4 also shows coincident localization with the MH4 IF in other structures such as the touch neuron channels, further supporting that MUP-4 associates with IFs. | |||
| Type | Reporter_gene | |||
| Pattern | GFP fluorescence was abundant in circumferential annular rings overlying muscle of 3 fold embryos. The antipeptide antibody raised to the intracellular domain showed similar staining. In contrast, this pattern was never observed in mutants; although mutant and wild-type (WT) embryos also exhibit diffuse patterns of antibody reactivity. | |||
| MUP-4 continues to localize to circumferential rings over body wall muscle in larvae and adults. MUP-4:: GFP and MUP-4 antibodies also localize to other regions where cells show mechanical attachments to the hypodermis including the inner surface of the pharynx where the radial pharyngeal muscle cells attach to the marginal hypodermal cells, which secrete a cuticle; overlying anal and intestinal muscles; overlying vulval and uterine sex muscles; male tail muscle attachment zones; and the six mechanosensory neurons that mediate touch sensitivity through their attachment to the hypodermis. Examination of WT animals also labeled with mAb MH27, which recognizes adherens junctions, shows that MUP-4 staining can extend beyond the muscle to the seam cell boundary. In some mup-4::gfp-expressing lines, high concentrations of protein was often seen around the hypodermal or touch neuron nuclei. | ||||
| MUP-4 continues to localize to circumferential rings over body wall muscle in larvae and adults. MUP-4::GFP and MUP-4 antibodies also localize to other regions where cells show mechanical attachments to the hypodermis including the inner surface of the pharynx where the radial pharyngeal muscle cells attach to the marginal hypodermal cells, which secrete a cuticle; overlying anal and intestinal muscles; overlying vulval and uterine sex muscles; male tail muscle attachment zones; and the six mechanosensory neurons that mediate touch sensitivity through their attachment to the hypodermis. Examination of WT animals also labeled with mAb MH27, which recognizes adherens junctions, shows that MUP-4 staining can extend beyond the muscle to the seam cell boundary. In some mup-4::gfp-expressing lines, high concentrations of protein was often seen around the hypodermal or touch neuron nuclei. | ||||
| Picture | WBPicture0000008942 | |||
| WBPicture0000008943 | ||||
| Reference | WBPaper00004798 | |||
| Transgene | WBTransgene00027594 |
