WormBase Tree Display for Expr_pattern: Expr1735
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| Expr1735 | Expression_of | Gene | WBGene00003870 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003870 | |||
| Expression_data | Life_stage | WBls:0000005 | ||
| Anatomy_term | WBbt:0004015 | Certain | ||
| WBbt:0004422 | Certain | |||
| WBbt:0005906 | Certain | |||
| WBbt:0005994 | Certain | |||
| WBbt:0006873 | Certain | |||
| WBbt:0006874 | Certain | |||
| WBbt:0006876 | Certain | |||
| GO_term | GO:0005886 | |||
| GO:0005819 | ||||
| GO:0005818 | ||||
| GO:0005783 | ||||
| GO:0048471 | ||||
| GO:0000922 | ||||
| Subcellular_localization | In early one-cell interphase and prophase embryos, OOC-5 was enriched around the rim of both pronuclei, as well as being dispersed throughout the cytoplasm in a slightly reticular pattern. A similar distribution was seen in two-cell interphase and prophase embryos. Double labeling with antibodies against tubulin showed that regions of more concentrated OOC-5 staining corresponded to the areas of the microtubule asters. During metaphase in one- and two-cell embryos, OOC-5 remained perinuclear and was also associated with the poles of the mitotic spindle. Upon onset of anaphase, OOC-5 localized on the entire mitotic spindle. The transition from perinuclear OOC-5 localization to general spindle localization coincides precisely with nuclear envelope breakdown in C. elegans. Similar patterns of OOC-5 localization were observed in four-cell embryos, depending on the stage of the cell cycle. In addition, OOC-5 was enriched at the regions of cell-cell contact in some two-cell embryos (40%, n=41) and virtually all four-cell embryos examined (96%, n=24). | |||
| Type | Antibody | Polyclonal rabbit antisera against the C-terminal region of the OOC-5 protein. On western blots, affinity-purified OOC-5 antibodies recognize a band of approximately 41 kDa in extracts of wild-type egg-bearing hermaphrodites, which is absent from protein extracts of ooc-5(it145) mutant worms. | ||
| Pattern | OOC-5 localization was observed in all cells of early wild-type embryos, in a pattern that changed with the cell cycle. | |||
| Picture | WBPicture0000007482 | |||
| WBPicture0000007484 | ||||
| Remark | early embryo (author) = blastula embryo (curator) --wjc. | |||
| Reference | WBPaper00005005 | |||
| Antibody_info | WBAntibody00000435 |
