WormBase Tree Display for Expr_pattern: Expr1866

Expr1866 Expression_of: Gene: WBGene00001410
    Reflects_endogenous_expression_of: WBGene00001410
  Homol: Homol_homol: Y54F10AM:Expr
  Expression_data: Life_stage: WBls:0000024
      WBls:0000038
      WBls:0000027
      WBls:0000035
      WBls:0000041
      WBls:0000019
      WBls:0000020
      WBls:0000021
    Anatomy_term: WBbt:0003681 Certain
      WBbt:0005300 Certain
      WBbt:0006749 Certain
      WBbt:0006759 Certain
    GO_term: GO:0043005
  Subcellular_localization: The GFP clearly outlines the shape of the expressing neurons with their processes (e.g. in the ventral nerve chord).
  Type: Reporter_gene
  Pattern: Expression of reporters confirmed that the main site of synthesis is the pharynx, but it is also expressed in some neurons in the nerve ring, in the ventral chord and in the tail. During embryogenesis, the expression of the constructs is detectable unambiguously only after proliferation has ceased and the embryo has elongated to the two- to three-fold stage. Expression of the reporters does not change substantially from embryogenesis and hatching to larval stages and adults. Expression is strong in several neurons and in pharyngeal cells. The very strong GFP signal detected in the pharynx did not allow the identification of feh-1-expressing cells among those forming this organ. The analysis of nuclear-targeted beta-galactosidase, expressed under the control of the feh-1 gene promoter, clearly identified, in agreement with GFP reporter activity, the nuclei of some extrapharyngeal neurons, as well as neurons of the ventral nerve chord and of the tail. Furthermore, in the procorpus and in the first pharyngeal bulb, the nuclei of m3 and m4 muscle cells are also stained. The staining of the nuclei in the second bulb, where the density is higher, did not unambiguously reveal the identity of feh-1-expressing cells.
  Picture: WBPicture0000009031
  Reference: WBPaper00005169
  Transgene: WBTransgene00027730