WormBase Tree Display for Expr_pattern: Expr1887
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| Expr1887 | Expression_of | Gene | WBGene00004215 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004215 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000010 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0003679 | Certain | ||
| WBbt:0005733 | Certain | |||
| WBbt:0005735 | Certain | |||
| WBbt:0007028 | Certain | |||
| GO_term | GO:0005912 | |||
| Subcellular_localization | PTP-3B::GFP was expressed in many but not all neurons, within which it was localized to neurites. In later embryos and larvae PTP-3B-GFP became localized within epidermal cells, apparently to adherens junctions. | |||
| Type | Antibody | Rabbit polyclonal antibodies against the intracellular domain of PTP-3; these antisera are expected to recognize both PTP-3 isoforms. | ||
| Reporter_gene | ||||
| Pattern | Antibody: The staining of anti-PTP-3 antisera in wild-type animals was weaker, but otherwise identical in pattern to the expression pattern of the PTP-3B::GFP transgenes. | |||
| Reporter_gene: PTP-3B::GFP transgenes showed widespread expression in embryos. The earliest stage GFP was detected in these embryos was during late gastrulation (approximately 250-300 minutes after first cleavage at 20C). PTP-3B::GFP expression was observed uniformly on the surface of most, possibly all, cells in the embryo during gastrulation cleft closure and epidermal enclosure. In later embryos, larvae and adults PTP-3B::GFP expression became highest in the nervous system, including the nerve ring, dorsal cord, and ventral cord. | ||||
| Reporter_gene: The Pptp-3A::GFP construct expressed GFP from the comma stage (380 minutes) onwards in many neurons that also expressed PTP-3B::GFP. Thus, PTP-3 isoforms are expressed in many tissues during early development, and later become restricted to the nervous system and epithelial tissues. | ||||
| Picture | WBPicture0000007504 | |||
| WBPicture0000007505 | ||||
| WBPicture0000007506 | ||||
| Reference | WBPaper00005259 | |||
| Transgene | WBTransgene00027737 | |||
| WBTransgene00031651 | ||||
| Antibody_info | WBAntibody00000509 |
