WormBase Tree Display for Expr_pattern: Expr2221
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| Expr2221 | Expression_of | Gene | WBGene00000383 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000383 | ||
| Expression_data | Life_stage | WBls:0000003 | |
| GO_term | GO:0030496 | ||
| GO:0005819 | |||
| Subcellular_localization | During interphase and early mitosis, antiCeCDC-14 antibodies produced only weak diffuse staining, but no labeling of specific cellular structures. However, in anaphase, striking staining of the central spindle area was observed in anaphase. Later in mitosis, during telophase, this staining compacted to a single dot that was positioned between the two daughter cells, highly reminiscent of the midbody. Older embryos displayed multiple dots in the cytoplasm or between cells, again consistent with midbody staining. Attesting to the specificity of this staining, neither the preimmune serum nor the affinity-purified serum preincubated with recombinant CeCDC-14 produced any distinct labeling. No staining of centrosomes or nuclei could be seen. Worms expressing a functional ZEN-4GFP fusion protein were stained with antibodies against GFP and CeCDC-14. The two antigens displayed extensive colocalization at both the central spindle and the polar body. | ||
| Type | Antibody | A polyclonal rabbit antibody was raised against a COOH-terminal fragment of CeCDC-14 (spanning amino acids 352-605). | |
| Pattern | The localization of CeCDC-14 was analyzed in wild-type C. elegans embryos, using the affinity-purified anti-CeCDC-14 antibodies for immunofluorescence microscopy. | ||
| Picture | WBPicture0000008957 | ||
| Remark | Expression pattern in other tissues or life stages are not described. | ||
| Reference | WBPaper00005453 | ||
| Antibody_info | WBAntibody00000546 |
