WormBase Tree Display for Expr_pattern: Expr2279
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| Expr2279 | Expression_of | Gene | WBGene00001511 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001511 | ||
| Expression_data | Life_stage | WBls:0000003 | |
| GO_term | GO:0005737 | ||
| GO:0000793 | |||
| Subcellular_localization | Using affinity-purified antibodies, FZY-1 was detected in early-stage embryonic cells at prometaphase and metaphase of mitosis and meiosis I. At prometaphase, highly condensed chromosomes were seen, and FZY-1 was localized around condensed chromosomes. At metaphase, FZY-1 localized along chromosomes aligned at the metaphase plate. After the separation of sister chromatids, the FZY-1 signal disappeared from the chromosomes, and at anaphase and interphase, cytoplasmic staining was observed. FZY-1 was observed around the meiotic chromosomes in cells at metaphase of meiosis I, but no signal was detected in cells at anaphase of meiosis I or meiosis II. The FZY-1 signal in cells at prometaphase or metaphase was not detected when antibodies were preincubated with purified recombinant FZY-1 protein, a finding that confirms the specificity of the antibodies. | ||
| Type | Antibody | Polyclonal antibodies to FZY-1. | |
| Pattern | Expressed in early-stage embryonic cells. No details on expression pattern in other life stages. | ||
| Picture | WBPicture0000009730 | ||
| Reference | WBPaper00005604 | ||
| Antibody_info | WBAntibody00000592 |
