WormBase Tree Display for Expr_pattern: Expr2280
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| Expr2280 | Expression_of | Gene | WBGene00006801 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006801 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| WBls:0000018 | |||
| WBls:0000019 | |||
| WBls:0000020 | |||
| WBls:0000017 | |||
| WBls:0000021 | |||
| Anatomy_term (13) | |||
| Type | Antibody | Polyclonal rabbit peptide antibody. | |
| Pattern | The R16 antibody stained muscle cells of wild-type from the comma stage to the 3-fold stage, but not those of x14 animals. The R16 antibody staining appeared at the later comma stage and became strong at the 1.5-fold stage, when the muscle starts twitching, and continued to the adult stage. Although background staining from the early embryo to pre-comma stages was also observed in the cytoplasm of whole cells, this staining was not different between wild-type and x14 animals. | ||
| The R16 antibody stained the body wall, pharyngeal, vulval and the anal muscles of adult hermaphrodites and male tail muscles in wild-type, but not in unc-68(x14) animals. The identity of these muscle tissues was confirmed by double-staining with rhodaminephalloidin. Among the anal muscles, the R16 antibody stained the depressor muscle but did not stain the sphincter muscle. The R16 antibody also stained pharyngeal muscles, especially those in the terminal bulb and isthmus. The staining pattern in body wall muscle was observed to be of a series of small dots. Because the antiserum stained in the intestines of both wild-type and unc-68(x14) animals, the intestinal staining is likely to represent the cross-reaction of the R16 antibody with unknown proteins. | |||
| Picture | WBPicture0000010568 | ||
| WBPicture0000010571 | |||
| Remark | The tissue-specific expression of unc-68 protein suggested by antibody staining was confirmed by a gfp-fusion construct. The results of unc-68::gfp expression and immunostaining experiments strongly suggest that CeRyR is present only in muscles. See Expr2281 for GFP result. | ||
| Reference | WBPaper00005595 | ||
| Antibody_info | WBAntibody00000584 |
