WormBase Tree Display for Expr_pattern: Expr2305

Expr2305 Expression_of: Gene: WBGene00003172
    Reflects_endogenous_expression_of: WBGene00003172
  Expression_data: Life_stage: WBls:0000024
      WBls:0000038
      WBls:0000027
      WBls:0000035
      WBls:0000041
      WBls:0000010
      WBls:0000013
    Anatomy_term: WBbt:0005734 Certain
    GO_term: GO:0005634
  Subcellular_localization: nuclei
  Type: Reporter_gene
  Pattern: GFP was detected in larval hyp7 nuclei at levels reduced from those seen in embryonic hypodermis and was not detected in larval body muscle cells. There were some differences between the antibody and GFP results: first, GFP was not reliably detected in the nuclei of ALM and AVM; and second, the nuclei of ventral hypodermal cells had detectable levels of GFP in young (L1-L2 stage) larvae but did not appear to stain with anti-MEC-8 antibodies.
    The pattern of GFP expression by transgenic embryos carrying mec-8::gfp was very similar to the pattern of MEC-8 expression seen by immunolocalization. GFP was seen in most nuclei at about the 50-cell stage. Just prior to morphogenesis, GFP became brighter in hypodermal nuclei and faded in the nuclei of other cells. During embryonic elongation, hypodermal nuclei exhibited bright GFP fluorescence while other nuclei fluoresced faintly or not at all. The nuclei of hypodermal cells and their precursor cells were marked by staining with anti-LIN-26. The only difference between the GFP expression and the anti-MEC-8 staining was that the faint expression seen in non-hypodermal nuclei carrying mec-8::gfp was not detected with anti-MEC-8 serum. This difference could have been caused by overexpression or perdurance of the MEC-8::GFP fusion protein or by poor antibody sensitivity to low levels of MEC-8.
  Picture: WBPicture0000007521
  Remark: See Expr2304 for antibody staining data.
  Reference: WBPaper00005568
  Transgene: WBTransgene00027990