WormBase Tree Display for Expr_pattern: Expr2308
expand all nodes | collapse all nodes | view schema
Expr2308 | Expression_of | Gene | WBGene00003916 | |
---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00003916 | |||
Expression_data | Life_stage | WBls:0000038 | ||
WBls:0000035 | ||||
WBls:0000057 | ||||
Anatomy_term | WBbt:0006748 | Certain | ||
GO_term | GO:0005938 | |||
Subcellular_localization | PAR-1 distribution was examined from the mid-L3 stage to young adulthood, using AJM-1 as a marker for the identity and position of vulval cells through vulval development. PAR-1 is localized near apical junctions prior to vulval development. This localization is common to other C. elegans epithelial cells. After fate specification and precursor proliferation, PAR-1 is not only found near apical junctions, but it is also associated with the cortex of the vulval cells. Through morphogenesis, PAR-1 gradually undergoes a redistribution from near apical junctions to the basolateral cortex. By young adulthood, PAR-1 is highly enriched in basolateral domains and little, if any, is still found associated with junctions. The PAR-1 distribution was identical using three different sources of antibodies to PAR-1 and, was sensitive to par-1(RNAi). | |||
Type | Antibody | Anti-PAR-1 polyclonal antibodies. Three antisera to PAR-1 produced identical staining patterns in the vulva of developing larvae. These included antiserum raised against a portion of the linker region(see Expr578) and two antisera raised against overlapping portions of the carboxyl terminus. | ||
Reference | WBPaper00005653 | |||
Antibody_info | WBAntibody00000096 | |||
WBAntibody00000598 |