WormBase Tree Display for Expr_pattern: Expr2406
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| Expr2406 | Expression_of | Gene | WBGene00006914 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006914 | |||
| Expression_data | Life_stage | WBls:0000003 | ||
| WBls:0000023 | ||||
| Anatomy_term | WBbt:0005812 | Certain | ||
| GO_term | GO:0016471 | |||
| Subcellular_localization | VHA-5 was detectable in a dot-like compartment in every embryonic cell. The compartment was found exclusively around the nucleus and observed during embryogenesis. A cup-shaped structure was often observed around nuclei instead of the dot-like compartment. On the other hand, VHA-5 expression changed dramatically at the larval stage and became dominant in an H-shaped excretory cell, consistent with the results for transgenic worms carrying the vha-5::GFP fusion gene. See Expr2403 for vha-5 GFP analysis. | |||
| Type | Antibody | Polyconal rabbit antibody. A 510-bp (Met-1 to Val-170 of VHA-5) cDNA fragment was inserted into pGEX6P-2(Amersham Pharmacia Biotech). The recombinant proteins expressed in Escherichia coli were used to immunize rabbits, and the antisera were affinity-purified using the same recombinant protein. | ||
| Picture | WBPicture0000008167 | |||
| Reference | WBPaper00004842 | |||
| Antibody_info | WBAntibody00000405 |
