WormBase Tree Display for Expr_pattern: Expr2462
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| Expr2462 | Expression_of | Gene | WBGene00003917 |
|---|---|---|---|
| WBGene00003921 | |||
| Reflects_endogenous_expression_of | WBGene00003917 | ||
| WBGene00003921 | |||
| Expression_data | Life_stage | WBls:0000003 | |
| GO_term | GO:0005737 | ||
| GO:0005813 | |||
| GO:0005938 | |||
| Subcellular_localization | Both GFP:PAR-2 and GFP:PAR-6 could be detected readily in the cytoplasm, and were also visibly enriched at the cortex. The first asymmetry was seen after the appearance of pronuclei when ruffling ceased abruptly near the paternal pronucleus. At that time, GFP:PAR-2 began to increase and GFP:PAR-6 began to decrease on the cortex in the smooth region. For the next 10 minutes, as the smooth region expanded towards the anterior, the GFP:PAR-2 domain expanded along with it, whereas GFP:PAR-6 receded, becoming more prominent in the area where ruffling is maintained. By pseudocleavage, the fusions reached their final configurations on the cortex, with GFP:PAR-2 enriched in the posterior and GFP:PAR-6 enriched in the anterior. GFP:PAR-2 was also detected on centrosomes during pronuclear rotation and GFP:PAR-6 was detected in both pronuclei just before pronuclear fusion. The latter localizations have not been reported previously for the endogenous proteins. | ||
| Type | Reporter_gene | ||
| Pattern | Before pronuclear formation, GFP:PAR-2 and GFP:PAR-6 were distributed uniformly throughout the embryo. | ||
| Picture | WBPicture0000007533 | ||
| WBPicture0000007534 | |||
| Reference | WBPaper00005735 | ||
| Transgene | WBTransgene00000082 | ||
| WBTransgene00000667 |
