WormBase Tree Display for Expr_pattern: Expr2543
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| Expr2543 | Expression_of | Gene | WBGene00006794 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006794 | ||||
| Expression_data | Life_stage | WBls:0000024 | |||
| WBls:0000003 | |||||
| WBls:0000038 | |||||
| WBls:0000027 | |||||
| WBls:0000035 | |||||
| WBls:0000041 | |||||
| Anatomy_term | WBbt:0005319 | Certain | |||
| WBbt:0005772 | Certain | ||||
| WBbt:0005784 | Partial | ||||
| Remark | Expressed in germ cells in the distal gonad and the oocytes. | ||||
| WBbt:0005813 | Certain | ||||
| WBbt:0006748 | Certain | ||||
| WBbt:0006749 | Certain | ||||
| WBbt:0006797 | Certain | ||||
| GO_term | GO:0005737 | ||||
| Subcellular_localization | At the one-cell stage, UNC-60A was diffuse in the cytoplasm and localized to no particular structure. Actin was localized throughout the cortex as well as in the diffuse cytoplasm and assembled at the early cleavage site in the telophase of the first mitosis, whereas UNC-60A did not significantly accumulate in these structures. During progression of cytokinesis and after the two-cell stage, association of UNC-60A with the cortical regions, especially at the cell-cell boundaries became evident. In addition, UNC-60A was colocalized with actin to dot-like structures at the anterior cortex of each blastomere. These are believed to be remnants of cell division. UNC-60B is diffusely localized in the cytoplasm of the embryonic body wall muscle in the later stages. | ||||
| In adult worms, localization of the nuclei of the gonad by DAPI indicated that both UNC-60A and UNC-60B were mostly localized to the cytoplasm. Also, they were undetectable in sperm, which does not have an actomyosin system. | |||||
| Type | Antibody | Rabbit polyclonal anti-UNC-60A and UNC-60B antibodies. Expression and localization of UNC-60A and UNC-60B were characterized by immunofluorescent staining of wild-type C. elegans with specific antibodies. | |||
| Pattern | In adult worms, UNC-60A was highly expressed in the intestine, germ cells in the distal gonad, oocytes and spermatheca but not in body wall muscle. UNC-60B was expressed in body wall muscle, vulva and spermatheca. Both ADF/cofilin isoforms were not detected in the smooth-muscle-like myoepithelial sheath cells. | ||||
| It was previously demonstrated that UNC-60B is not expressed in early embryos and become detectable from the comma to 1.5-fold stages (approximately 290 minutes after first cell division) only in the body wall muscle cells. | |||||
| UNC-60A was maternally expressed and present in most of embryonic cells throughout embryogenesis. In the later stages, staining of UNC-60A in the nerve ring became intense. | |||||
| Picture | WBPicture0000009039 | ||||
| WBPicture0000009040 | |||||
| WBPicture0000009041 | |||||
| Reference | WBPaper00005824 | ||||
| Antibody_info | WBAntibody00000222 | ||||
| WBAntibody00000223 |
